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Book ; Online ; Thesis: Wachstum von respiratorischen Epithelzellen unter Einfluss von porcinem Surfactant in vitro

Wemhöner, Andreas

2004

Author's details	vorgelegt von Andreas Wemhöner
Language	German
Size	Online-Ressource
Document type	Book ; Online ; Thesis
Thesis / German Habilitation thesis	Univ., Diss--Bochum, 2004
Database	Former special subject collection: coastal and deep sea fishing

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Article ; Online: Pulmonary surfactant preserves viability of alveolar type II cells exposed to polymyxin B in vitro.

Stichtenoth, Guido / Herting, Egbert / Rüdiger, Mario / Wemhöner, Andreas

PloS one

2013 Volume 8, Issue 4, Page(s) e62105

Abstract: Background: Exogenous surfactant derived from animal lungs is applied for treatment of surfactant deficiency. By means of its rapid spreading properties, it could transport pharmaceutical agents to the terminal air spaces. The antimicrobial peptide ... ...

Abstract	Background: Exogenous surfactant derived from animal lungs is applied for treatment of surfactant deficiency. By means of its rapid spreading properties, it could transport pharmaceutical agents to the terminal air spaces. The antimicrobial peptide Polymyxin B (PxB) is used as a topical antibiotic for inhalation therapy. Whereas it has been shown that PxB mixed with surfactant is not inhibiting surface activity while antimicrobiotic activity is preserved, little is known concerning the effects on synthesis of endogenous surfactant in alveolar type II cells (ATIIC). Objective: To investigate ATIIC viability and surfactant-exocytosis depending on PxB and/or surfactant exposure. Methods: ATIIC were isolated from rat lungs as previously described and were cultivated for 48 h. After incubation for a period of 1-5 h with either PxB (0.05 or 0.1 mg/ml), modified porcine surfactant (5 or 10 mg/ml) or mixtures of both, viability and exocytosis (spontanously and after stimulation) were determined by fluorescence staining of intracellular surfactant. Results: PxB 0.1 mg/ml, but not porcine surfactant or porcine surfactant plus PxB reduces ATIIC-viability. Only PxB alone, but not in combination with porcine surfactant, rapidly reduces fluorescence in ATIIC at maximum within 3 h, indicating stimulation of exocytosis. Subsequent ionomycin-stimulation does not further increase exocytosis of PxB incubated ATIIC. In presence of surfactant, stimulating effects of PxB and ionomycin on exocytosis are reduced. Conclusion: PxB alone shows negative effects on ATIIC, which are counterbalanced in mixtures with surfactant. So far, our studies found no results discouraging the concept of a combined treatment with PxB and surfactant mixtures.
MeSH term(s)	Alveolar Epithelial Cells/cytology ; Alveolar Epithelial Cells/drug effects ; Animals ; Cell Survival/drug effects ; Exocytosis/drug effects ; Ionomycin/pharmacology ; Polymyxin B/pharmacology ; Pulmonary Surfactants/pharmacology ; Rats ; Rats, Sprague-Dawley ; Sus scrofa
Chemical Substances	Pulmonary Surfactants ; Ionomycin (56092-81-0) ; Polymyxin B (J2VZ07J96K)
Language	English
Publishing date	2013-04-19
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1932-6203
ISSN (online)	1932-6203
DOI	10.1371/journal.pone.0062105
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Book ; Online ; Thesis: Wachstum von respiratorischen Epithelzellen unter Einfluss von porcinem Surfactant in vitro

Wemhöner, Andreas [Verfasser]

2004

Author's details	vorgelegt von Andreas Wemhöner
Keywords	Medizin, Gesundheit ; Medicine, Health
Subject code	sg610
Document type	Book ; Online ; Thesis
Database	Digital theses on the web

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Article ; Online: Nutrition of preterm infants in relation to bronchopulmonary dysplasia

Tschirch Edda / Ortner Daniel / Wemhöner Andreas / Strasak Alexander / Rüdiger Mario

BMC Pulmonary Medicine, Vol 11, Iss 1, p

2011 Volume 7

Abstract: Background The pathogenesis of bronchopulmonary dysplasia (BPD) is multifactorial. In addition to prenatal inflammation, postnatal malnutrition also affects lung development. Methods A retrospective study was performed to analyse during the first two ... ...

Abstract	Background The pathogenesis of bronchopulmonary dysplasia (BPD) is multifactorial. In addition to prenatal inflammation, postnatal malnutrition also affects lung development. Methods A retrospective study was performed to analyse during the first two weeks of life the total, enteral and parenteral nutrition of premature infants (<31 weeks, birth weight ≤1500 g) born between 08/04 and 12/06. Results Ninety-five premature infants were analysed: 26 with BPD (27 ± 1 weeks) and 69 without BPD (28 ± 1 weeks). There was no statistical significant difference in the total intake of fluids, calories, glucose or protein and weight gain per day in both groups. The risk of developing BPD was slightly increased in infants with cumulative caloric intake below the minimal requirement of 1230 kcal/kg and a cumulative protein intake below 43.5 g/kg. Furthermore, the risk of developing BPD was significantly higher when infants had a cumulative fluid intake above the recommended 1840 ml/kg. In infants who developed BPD, the enteral nutrition was significantly lower than in non-BPD infants [456 ml/kg (IQR 744, 235) vs. 685 (IQR 987, 511)]. Infants who did not develop BPD reached 50% of total enteral feeding significantly faster [9.6 days vs. 11.5]. Conclusions Preterm infants developing BPD received less enteral feeding, even though it was well compensated by the parenteral nutrient supply. Data suggest that a critical minimal amount of enteral feeding is required to prevent development of BPD; however, a large prospective clinical study is needed to prove this assumption.
Keywords	Internal medicine ; RC31-1245 ; Medicine ; R ; DOAJ:Internal medicine ; DOAJ:Medicine (General) ; DOAJ:Health Sciences
Subject code	610
Language	English
Publishing date	2011-02-01T00:00:00Z
Publisher	BioMed Central
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Effect of exogenous surfactants on viability and DNA synthesis in A549, immortalized mouse type II and isolated rat alveolar type II cells

Haller Thomas / Jennings Paul / Wemhöner Andreas / Rüdiger Mario / Simbruner Georg

BMC Pulmonary Medicine, Vol 11, Iss 1, p

2011 Volume 11

Abstract: Abstract Background In mechanically ventilated preterm infants with respiratory distress syndrome (RDS), exogenous surfactant application has been demonstrated both to decrease DNA-synthesis but also and paradoxically to increase epithelial cell ... ...

Abstract	Abstract Background In mechanically ventilated preterm infants with respiratory distress syndrome (RDS), exogenous surfactant application has been demonstrated both to decrease DNA-synthesis but also and paradoxically to increase epithelial cell proliferation. However, the effect of exogenous surfactant has not been studied directly on alveolar type II cells (ATII cells), a key cell type responsible for alveolar function and repair. Objective The aim of this study was to investigate the effects of two commercially available surfactant preparations on ATII cell viability and DNA synthesis. Methods Curosurf ® and Alveofact ® were applied to two ATII cell lines (human A549 and mouse iMATII cells) and to primary rat ATII cells for periods of up to 24 h. Cell viability was measured using the redox indicator resazurin and DNA synthesis was measured using BrdU incorporation. Results Curosurf ® resulted in slightly decreased cell viability in all cell culture models. However, DNA synthesis was increased in A549 and rat ATII cells but decreased in iMATII cells. Alveofact ® exhibited the opposite effects on A549 cells and had very mild effects on the other two cell models. Conclusion This study showed that commercially available exogenous surfactants used to treat preterm infants with RDS can have profound effects on cell viability and DNA synthesis.
Keywords	Internal medicine ; RC31-1245 ; Medicine ; R ; DOAJ:Internal medicine ; DOAJ:Medicine (General) ; DOAJ:Health Sciences
Subject code	610
Language	English
Publishing date	2011-02-01T00:00:00Z
Publisher	BioMed Central
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Inflammatory cytokine mRNA in monocytes is modified by a recombinant (SP-C)-based surfactant and porcine surfactant.

Wemhöner, Andreas / Rüdiger, Mario / Gortner, Ludwig

Methods and findings in experimental and clinical pharmacology

2009 Volume 31, Issue 5, Page(s) 317–323

Abstract: Respiratory distress syndrome (RDS) is mainly caused by a deficiency of surfactant in structurally immature lungs. Therapy for RDS consists of mechanical ventilation and administration of exogenous surfactant. Animal-derived surfactant preparations that ... ...

Abstract	Respiratory distress syndrome (RDS) is mainly caused by a deficiency of surfactant in structurally immature lungs. Therapy for RDS consists of mechanical ventilation and administration of exogenous surfactant. Animal-derived surfactant preparations that are used to treat newborn infants show inhibition of proinflammatory cytokines. There are no data available concerning the effects of the new generation of surfactants. In the present study, the effects of an animal-derived surfactant (Curosurf) and a synthetic surfactant (Venticute) on lipopolysaccharide (LPS)-induced inflammation were tested in human monocyte THP-1 cells. The effects were measured as changes in messenger RNA (mRNA) expression of the chemokine interleukin-8 (IL-8), proinflammatory TNF-alpha and the anti-inflammatory IL-10 cytokine. Both surfactant preparations inhibited the LPS-induced increase in TNF-alpha expression. A comparison of both preparations revealed a similar effect on IL-10 expression. However, IL-10 expression was higher after incubation with Venticute. Curosurf increased IL-8 expression at higher concentrations, but Venticute had no effect. The anti-inflammatory effect of an animal-derived surfactant and a new-generation synthetic surfactant preparation may influence postnatal pulmonary inflammation.
MeSH term(s)	Biological Products/pharmacology ; Cells, Cultured ; Cytokines/genetics ; Humans ; Interleukin-10/biosynthesis ; Interleukin-8/genetics ; Lipopolysaccharides/pharmacology ; Monocytes/metabolism ; Phospholipids/pharmacology ; RNA, Messenger/analysis ; Recombinant Proteins/pharmacology ; Tumor Necrosis Factor-alpha/genetics
Chemical Substances	Biological Products ; Cytokines ; Interleukin-8 ; Lipopolysaccharides ; Phospholipids ; RNA, Messenger ; Recombinant Proteins ; Tumor Necrosis Factor-alpha ; Venticute ; Interleukin-10 (130068-27-8) ; poractant alfa (KE3U2023NP)
Language	English
Publishing date	2009-06
Publishing country	Spain
Document type	Journal Article
ZDB-ID	446847-8
ISSN	2013-0155 ; 0379-0355
ISSN (online)	2013-0155
ISSN	0379-0355
DOI	10.1358/mf.2009.31.5.1380462
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Effect of exogenous surfactants on viability and DNA synthesis in A549, immortalized mouse type II and isolated rat alveolar type II cells.

Wemhöner, Andreas / Jennings, Paul / Haller, Thomas / Rüdiger, Mario / Simbruner, Georg

BMC pulmonary medicine

2011 Volume 11, Page(s) 11

Abstract: Background: In mechanically ventilated preterm infants with respiratory distress syndrome (RDS), exogenous surfactant application has been demonstrated both to decrease DNA-synthesis but also and paradoxically to increase epithelial cell proliferation. ... ...

Abstract	Background: In mechanically ventilated preterm infants with respiratory distress syndrome (RDS), exogenous surfactant application has been demonstrated both to decrease DNA-synthesis but also and paradoxically to increase epithelial cell proliferation. However, the effect of exogenous surfactant has not been studied directly on alveolar type II cells (ATII cells), a key cell type responsible for alveolar function and repair. Objective: The aim of this study was to investigate the effects of two commercially available surfactant preparations on ATII cell viability and DNA synthesis. Methods: Curosurf® and Alveofact® were applied to two ATII cell lines (human A549 and mouse iMATII cells) and to primary rat ATII cells for periods of up to 24 h. Cell viability was measured using the redox indicator resazurin and DNA synthesis was measured using BrdU incorporation. Results: Curosurf® resulted in slightly decreased cell viability in all cell culture models. However, DNA synthesis was increased in A549 and rat ATII cells but decreased in iMATII cells. Alveofact® exhibited the opposite effects on A549 cells and had very mild effects on the other two cell models. Conclusion: This study showed that commercially available exogenous surfactants used to treat preterm infants with RDS can have profound effects on cell viability and DNA synthesis.
MeSH term(s)	Animals ; Biological Products/pharmacology ; Cell Line ; Cell Proliferation/drug effects ; Cell Survival/drug effects ; Cells, Cultured ; DNA/metabolism ; Epithelial Cells/cytology ; Epithelial Cells/drug effects ; Epithelial Cells/metabolism ; Humans ; Male ; Mice ; Models, Animal ; Phospholipids/pharmacology ; Pulmonary Alveoli/cytology ; Pulmonary Alveoli/drug effects ; Pulmonary Alveoli/metabolism ; Pulmonary Surfactants/pharmacology ; Rats ; Rats, Sprague-Dawley
Chemical Substances	Biological Products ; Phospholipids ; Pulmonary Surfactants ; SF-RI 1, bovine surfactant preparation ; DNA (9007-49-2) ; poractant alfa (KE3U2023NP)
Language	English
Publishing date	2011-02-17
Publishing country	England
Document type	Journal Article
ZDB-ID	2059871-3
ISSN	1471-2466 ; 1471-2466
ISSN (online)	1471-2466
ISSN	1471-2466
DOI	10.1186/1471-2466-11-11
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Nutrition of preterm infants in relation to bronchopulmonary dysplasia.

Wemhöner, Andreas / Ortner, Daniel / Tschirch, Edda / Strasak, Alexander / Rüdiger, Mario

BMC pulmonary medicine

2011 Volume 11, Page(s) 7

Abstract: Background: The pathogenesis of bronchopulmonary dysplasia (BPD) is multifactorial. In addition to prenatal inflammation, postnatal malnutrition also affects lung development.: Methods: A retrospective study was performed to analyse during the first ... ...

Abstract	Background: The pathogenesis of bronchopulmonary dysplasia (BPD) is multifactorial. In addition to prenatal inflammation, postnatal malnutrition also affects lung development. Methods: A retrospective study was performed to analyse during the first two weeks of life the total, enteral and parenteral nutrition of premature infants (<31 weeks, birth weight ≤1500 g) born between 08/04 and 12/06. Results: Ninety-five premature infants were analysed: 26 with BPD (27 ± 1 weeks) and 69 without BPD (28 ± 1 weeks). There was no statistical significant difference in the total intake of fluids, calories, glucose or protein and weight gain per day in both groups. The risk of developing BPD was slightly increased in infants with cumulative caloric intake below the minimal requirement of 1230 kcal/kg and a cumulative protein intake below 43.5 g/kg. Furthermore, the risk of developing BPD was significantly higher when infants had a cumulative fluid intake above the recommended 1840 ml/kg. In infants who developed BPD, the enteral nutrition was significantly lower than in non-BPD infants [456 ml/kg (IQR 744, 235) vs. 685 (IQR 987, 511)]. Infants who did not develop BPD reached 50% of total enteral feeding significantly faster [9.6 days vs. 11.5]. Conclusions: Preterm infants developing BPD received less enteral feeding, even though it was well compensated by the parenteral nutrient supply. Data suggest that a critical minimal amount of enteral feeding is required to prevent development of BPD; however, a large prospective clinical study is needed to prove this assumption.
MeSH term(s)	Bronchopulmonary Dysplasia/epidemiology ; Bronchopulmonary Dysplasia/etiology ; Energy Intake ; Enteral Nutrition ; Female ; Glucose ; Humans ; Infant ; Infant, Newborn ; Infant, Premature ; Male ; Nutrition Assessment ; Nutritional Requirements ; Parenteral Nutrition ; Proteins ; Retrospective Studies ; Risk Factors
Chemical Substances	Proteins ; Glucose (IY9XDZ35W2)
Language	English
Publishing date	2011-02-03
Publishing country	England
Document type	Journal Article
ZDB-ID	2059871-3
ISSN	1471-2466 ; 1471-2466
ISSN (online)	1471-2466
ISSN	1471-2466
DOI	10.1186/1471-2466-11-7
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Continuous noninvasive monitoring of lung recruitment during high-frequency oscillatory ventilation by electrical impedance measurement: an animal study.

Burkhardt, Wolfram / Kurth, Florian / Pitterle, Manuela / Blassnig, Nicola / Wemhöner, Andreas / Rüdiger, Mario

Neonatology

2013 Volume 103, Issue 3, Page(s) 218–223

Abstract: Background: Ventilatory pressures should target the range between the upper and lower inflection point of the pressure volume curve in order to avoid atelecto- and volutrauma. During high-frequency oscillatory ventilation (HFOV), this range is difficult ...

Abstract	Background: Ventilatory pressures should target the range between the upper and lower inflection point of the pressure volume curve in order to avoid atelecto- and volutrauma. During high-frequency oscillatory ventilation (HFOV), this range is difficult to determine. Quadrant impedance measurement (QIM) has recently been shown to allow accurate and precise measurement of lung volume changes during conventional mechanical ventilation. Objectives: To investigate if QIM can be used to determine a static pressure-residual impedance curve during a recruitment-derecruitment manoeuvre on HFOV and to monitor the time course of alveolar recruitment after changing mean airway pressure (MAP). Methods: An incremental and decremental MAP trial (6 cm H2O to 27 cm H2O) was conducted in five surfactant-depleted newborn piglets during HFOV. Ventilatory, gas exchange and haemodynamic parameters were recorded. Continuous measurement of thoracic impedance change was performed. Results: Mean residual impedance (RI) increased with each stepwise increase of MAP resulting in a total mean increase of +26.5% (±4.0) at the highest MAP (27 cm H2O) compared to baseline ventilation at 6 cm H2O. Upon decreasing MAP levels, RI fell more slowly compared to its ascent; 83.4% (±19.1) and 84.8% (±16.4) of impedance changes occurred in the first 5 min after an increase or decrease in airway pressure, respectively. Conclusions: QIM could be used for continuous monitoring of thoracic impedance and determination of the pressure-RI curve during HFOV. The method could prove to be a promising bedside method for the monitoring of lung recruitment during HFOV in the future.
MeSH term(s)	Animals ; Animals, Newborn ; Blood Gas Analysis ; Electric Impedance ; Hemodynamics ; High-Frequency Ventilation ; Lung/physiology ; Lung Volume Measurements ; Monitoring, Physiologic/methods ; Pressure ; Pulmonary Ventilation ; Swine ; Time Factors
Language	English
Publishing date	2013
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2266911-5
ISSN	1661-7819 ; 1661-7800
ISSN (online)	1661-7819
ISSN	1661-7800
DOI	10.1159/000345612
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Effects of Perfluorocarbons on surfactant exocytosis and membrane properties in isolated alveolar type II cells

Ravasio Andrea / Hobi Nina / Hackspiel Irmgard / Wemhöner Andreas / Haller Thomas / Rüdiger Mario

Respiratory Research, Vol 11, Iss 1, p

2010 Volume 52

Abstract: Abstract Background Perfluorocarbons (PFC) are used to improve gas exchange in diseased lungs. PFC have been shown to affect various cell types. Thus, effects on alveolar type II (ATII) cells and surfactant metabolism can be expected, data, however, are ... ...

Abstract	Abstract Background Perfluorocarbons (PFC) are used to improve gas exchange in diseased lungs. PFC have been shown to affect various cell types. Thus, effects on alveolar type II (ATII) cells and surfactant metabolism can be expected, data, however, are controversial. Objective The study was performed to test two hypotheses: (I) the effects of PFC on surfactant exocytosis depend on their respective vapor pressures; (II) different pathways of surfactant exocytosis are affected differently by PFC. Methods Isolated ATII cells were exposed to two PFC with different vapor pressures and spontaneous surfactant exocytosis was measured. Furthermore, surfactant exocytosis was stimulated by either ATP, PMA or Ionomycin. The effects of PFC on cell morphology, cellular viability, endocytosis, membrane permeability and fluidity were determined. Results The spontaneous exocytosis was reduced by PFC, however, the ATP and PMA stimulated exocytosis was slightly increased by PFC with high vapor pressure. In contrast, Ionomycin-induced exocytosis was decreased by PFC with low vapor pressure. Cellular uptake of FM 1-43 - a marker of membrane integrity - was increased. However, membrane fluidity, endocytosis and viability were not affected by PFC incubation. Conclusions We conclude that PFC effects can be explained by modest, unspecific interactions with the plasma membrane rather than by specific interactions with intracellular targets.
Keywords	Diseases of the respiratory system ; RC705-779 ; Specialties of internal medicine ; RC581-951 ; Internal medicine ; RC31-1245 ; Medicine ; R ; DOAJ:Internal medicine ; DOAJ:Medicine (General) ; DOAJ:Health Sciences
Subject code	571
Language	English
Publishing date	2010-05-01T00:00:00Z
Publisher	BioMed Central
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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